RecruitingObservational

Role of Senescent Cells in Uterine Fibroid Pathogenesis

NCT ID: NCT06135870Sponsor: Johns Hopkins UniversityLast updated: 2025-12-11

Summary

The investigators are evaluating the role of senescent cells in uterine fibroids.

Detailed description

Uterine fibroids are prevalent tumors of uterus characterized by excessive fibrotic tissues. Using new cutting-edge computational methods, the investigators have found that small groups of senescent cells in fibroids work in concert with immune cells to produce soluble factors in fibroid tissues to create a feed-forward loop leading to fibrosis. This project seeks to unravel key cell-cell communication networks involving senescent and immune cells in fibroids to develop new treatments for uterine fibroids.

Arms & interventions

Outcome measures

Primary

Develop an atlas to comprehensively quantify the rate of senescent cell (SnC) types in fibroid and myometrium tissue samples
Perform scRNASeq on human fibroids and control myometrium tissue and apply transfer learning algorithm for SnC identification and phenotyping to identify cell-cell communication patterns in fibroids versus control myometrial tissue using Domino to validate computationally predicted senescent cell types. The computationally predicted immune phenotypes will be validated with flow cytometry.
Time frame: 5 years

Secondary

Validate the concentration senescence-associated secretory profile (SASP) in cell cultures of senescent cells in uterine fibroids
Time frame: 5 years
Rate of inhibition of cellular proliferation and ECM deposition by senolytics and senomorphics in fibroids
Time frame: 5 years

Eligibility criteria

Sex: FemaleAge: 18 Years to 55 YearsHealthy volunteers: No

Inclusion Criteria: * Subjects must be women above the age of ≥18 - ≤55 years. * Women with suspected uterine fibroids. * Subject must be or have been sexually active or have had a previous vaginal exam that used a speculum. * Subject must be able to understand, read and speak English or Spanish and subsequently, be able to understand what she is consenting to in terms of procedures and use/disclosure of protected health information. Exclusion Criteria: * Unwilling to sign the consent form or complete questionnaire. * Pregnancy. * Cancer of the uterus

Study locations (1)

Johns Hopkins Hospital

Baltimore, Maryland, 21287

Recruiting

Rachel Mitchel · Contact

rmiche10@jhmi.edu